The introduction of tags, whether short peptides (6X-His, DYKDDDDK, c-myc) or proteins (green fluorescent protein – GFP, glutathione-s-transferase – GST) into recombinant proteins aids in the characterization, localization or purification of the recombinant expressed protein.1-3 As a consequence, antibodies raised against fusion tags are useful in biochemical or immunohistochemistry applications.
AnaSpec, EGT Group is pleased to offer a wide selection of high quality monoclonal and polyclonal antibodies against the hexa-His and GFP tags.
Fluorescently labeled and biotin labeled 6X-His antibodies are also available. Antibodies are labeled with a selection of dyes, including our proprietary HiLyte Fluor™ dyes, which span the visible spectrum. This gives researchers a wide range of wavelength choices for use in immunohistochemistry or immunofluorescence applications.
References:
1. Zheng et al. J Biochem 133, 577 (2003).
2. Amrein et al. PNAS 92, 1048 (1995).
3. Nakajima K. and Yaoita Y. Nucleic Acids Res 25, 2231 (1997).
4. Medina et al. Nucleic Acids Res 28, I (2000).
For more information, please visit http://www.anaspec.com/products/promotions.asp?id=96&col=2&row=2
