The couple, who have been dating for around two-and-a-half years, are believed to be going through a rocky patch, but friends have denied claims they have already split.
A source said: Things have been a bit up in the air recently. They've been spending more time apart more time with each of their friends, and they've been re-evaluating their relationship.
Earlier this month, 27-year-old Jessica attended the Los Angeles wrap party for new movie Valentine’s Day alone. Justin, 28, was spotted partying with pals in West Hollywood days later.
Another source claimed: They're together. They're not breaking up. Any time they spend time apart it's because they're working.
Recent reports have claimed the pair split up to a month ago, but are not ready to go public with the news.
Us Weekly magazine claims: After nearly three rocky years, Justin Timberlake and Jessica Biel have called it quits.
A mutual pal of the pair tells us that Justin has told friends, It's over, and that he ended it for good - over the phone.
It has also been suggested Justin has grown close to singer Rihanna, 21, while they have been working on new music together.
The star’s close friendship reportedly put increased strain on his relationship with Jessica.
Justin and Jessica are currently in Santa Barbara together, and were spotted enjoying a romantic meal on Monday night (28.09.09).
A source told gossip website X17Online: They tried to fly under the radar when they sat down for their meal, but they certainly didn't act like a couple who had just broken up! Justin and Jessica held hands and gazed into each others eyes during dinner, and they seemed very relaxed. No signs of drama here!