Aminoluciferin (aLuc) is luciferin with its 6-position hydroxyl group substituted with an amino group. This modification allows aLuc to form peptide (amide) bonds with a peptide, while at the same time retaining the transport and bioluminescent properties of luciferin, resulting in a molecule called peptide-aminoluciferin.1
Many peptides-aminoluciferin are good substrates for luciferase-based (bioluminescence) assays. These substrates generally offer significant advantages, such as increased sensitivity, ease of use, and high throughput screening capacity. Luciferase-based assays are typically 10- to 100-fold more sensitive than comparable fluorescent assays (rhodamine 110, AMC and AFC).
AnaSpec now offers custom synthesis service of peptide-aminoluciferin substrates.
AnaSpec, Inc. is a leading provider of integrated proteomics solutions to pharmaceutical, biotech, and academic research institutions throughout the world. With a vision for innovation through synergy, AnaSpec focuses on three core technologies: peptides, detection reagents (dyes, assay kits, & antibodies), and combinatorial chemistry.
Reference:
1. Wilson, B. et al. (Ed.) Advances in Biophotonics. IOS Press, 2005.
