Colorimetric based Peptide-p-Nitroanilide conjugates (peptide-pNAs), with absorbance at approximately 408 nm, have historically been and are still widely used substrates for the study of protease activity. However, the preparation of peptide-pNA presents technical challenges. The amino group of pNA has a low nucleophilic property due to the electron-withdrawing effect of the nitro group; poor solubility of a p-nitroanilide intermediate and coupling in solution phase by DCC, azide or active ester, techniques commonly used are not effective.
Chemists at AnaSpec, Eurogentec Group have developed a Wang resin conjugated with a pNA analog, 5-amino-2-nitrozenzoic acid (Anb5,2). This novel support for facile solid phase pNA-like peptide substrate syntheses was developed based in part on a paper by Hojo, et al. in which they described the introduction of Anb5,2 to a p-methylbenzhydrylamine (MB) resin. The Wang preloaded resin is a useful tool for the synthesis of peptide with a C-terminal pNA- analog, Anb.
AnaSpec also have presented a poster at the recent ABRF meeting in San Antonio, Texas, entitled, “Facile Solid-Phase Synthesis of Peptide-7-p-Nitroanilide (pNA) Analog Containing Conjugates Using a Novel Wang or Rink Amide Resin.”
Reference:
Hojo, K. et al. Chem. Pharm. Bull. U.S.A. 48, 1740 (2000).
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AnaSpec, EGT Group:
AnaSpec, EGT Group is a leading provider of integrated proteomics and genomics solutionsTM for worldwide life science research. AnaSpec offers expertise in peptides, detection reagents, antibodies, assay kits, amino acids, oligonucleotides, and qPCRs. AnaSpec carries a broad product line of biochemicals and reagents for basic research, high-throughput screening and drug discovery. AnaSpec also provides premier custom services including peptide synthesis, antibody production, assay development, fluorescent dyes, and oligonucleotide synthesis. AnaSpec is certified to ISO 9001:2008.
